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Vitamin B3 Profile (Niacin Metabolism: Nicotinamide, Nicotinic Acid, NAD)
€ 35,00
Assesses vitamin B3 levels, crucial for metabolism and heart health.
Your test at a glance
- Sample
- Blood
- Results from receipt
- Usually 3–10 days
- Fasting
- Check your WHL instructions before collection
Timing starts when suitable samples arrive at the laboratory. Shipping time is additional. Combined panels may take longer.
Preparation: Pause non-prescribed nutritional supplements for at least 48 hours, unless WHL or your clinician advises otherwise.
WHL will send the full test instructions with your kit or appointment confirmation. Follow these before collecting.
Preparation and important notesOne charge per request. Processing and handling is charged once for the complete request, not for each test or panel. Collection and delivery charges are shown separately at checkout.
Description
Overview
Vitamin B3 (niacin) is central to cellular energy metabolism through its role in NAD⁺ and NADP⁺, which are essential cofactors in redox reactions, mitochondrial function, DNA repair, and cellular stress responses.
Because vitamin B3 functions primarily through these coenzymes, a functional assessment benefits from measuring multiple niacin-related compounds, rather than a single marker.
What This Test Measures
This profile includes:
- Nicotinamide
The predominant circulating form of vitamin B3 and a key NAD precursor. - Nicotinic acid
A dietary and supplemental form of niacin. - NAD (nicotinamide adenine dinucleotide)
A central coenzyme in energy production and redox balance. - Total vitamin B3 (expressed as nicotinamide equivalents)
Provides an integrated view of vitamin B3 availability and utilization.
Clinical Use
This profile is helpful for:
- Evaluating energy metabolism and mitochondrial function
- Investigating fatigue, cognitive symptoms, mood disturbances
- Assessing NAD⁺-related metabolic stress
- Monitoring niacin or NAD-precursor supplementation
Altered patterns may reflect increased metabolic turnover, impaired synthesis from tryptophan, or high cellular demand.
Method
Quantification is performed by LC–MS/MS.
Sample Requirement
Whole blood
Suitable for postal submission.
Additional information
| Weight | 0,5 kg |
|---|---|
| Dimensions | 25 × 16 × 2,5 cm |
| Sample Type |
Preparing for this test
WHL will send detailed collection, storage and return instructions with your test kit or appointment confirmation. Those test-specific instructions take priority.
Sample: Blood
Fasting: Check your WHL instructions before collection
For nutritional testing, pause non-prescribed supplements, including protein and amino-acid powders, for at least 48 hours unless WHL or your clinician advises otherwise. If monitoring supplements, agree the timing with your practitioner.
Do not stop prescribed medicines or prescribed supplements without advice from your treating clinician. Tell WHL about high-dose biotin; some tests need a longer pause. A short supplement pause does not remove all effects of long-term use.
If another test in the same order requires fasting, follow the combined instructions. Ask your clinician or WHL before fasting if you have diabetes, use glucose-lowering medicine, are pregnant, or are arranging testing for a child.
When to expect your results
Usually 3–10 days. This estimate starts when suitable samples reach the laboratory, not on the order or collection date. Shipping is additional. WHL will confirm any test-specific exception; a combined panel follows its slowest component.
Practical preparation tips
If fasting is requested, drink only plain water in your usual amounts. Do not have coffee, tea, juice, milk, energy drinks, sweets or chewing gum. Avoid smoking or vaping that morning. Book an early appointment and eat after collection.
For a planned blood test, avoid alcohol and unusually strenuous exercise for 24 hours, where practical, and sit quietly for about 15 minutes before collection. Tell WHL if you could not follow the preparation instructions.
Do not extend fasting or change prescribed medication on your own. Ask your clinician first if you have diabetes, need medicine with food, are pregnant, or are arranging testing for a child. WHL’s full test-specific instructions take priority.
Sources for this general preparation guidance
- Mayo Clinic Laboratories — plasma amino acids
- Mayo Clinic Laboratories — vitamin B6
- Mayo Clinic Laboratories — B12 and folate
- Mayo Clinic Laboratories — vitamins A and E
- Mayo Clinic Laboratories — iron studies
- NHS — fasting blood tests
- EAS/EFLM — fasting and non-fasting lipids
- EFLM/COLABIOCLI — preparation for blood collection
Other laboratories’ methods and preparation rules can differ. These sources support the general advice; WHL confirms the instructions for the method used for your test.




