Vitamin B1 Profile (Thiamine and Thiamine Diphosphate – TDP)

Beschrijving

Overview

Vitamin B1 (thiamine) is an essential water-soluble vitamin required for mitochondrial energy production, glucose metabolism, and neurological and cardiovascular function. Inside cells, thiamine is converted into phosphorylated forms, of which thiamine diphosphate (TDP / TPP) is the primary biologically active coenzyme.

Because circulating free thiamine alone does not reliably reflect intracellular availability, assessment of multiple thiamine forms provides a more accurate picture of functional vitamin B1 status.

What This Test Measures

This profile quantifies several vitamin B1 fractions in blood:

• Thiamine (free thiamine)
  Reflects recent intake and short-term availability.
• Thiamine monophosphate (TMP)
  An intermediate form in thiamine metabolism.
• Thiamine diphosphate (TDP / TPP)
  The metabolically active coenzyme form, primarily located inside erythrocytes and directly linked to enzymatic activity.
• Total vitamin B1 (expressed as TDP equivalents)
  Provides an integrated measure of overall vitamin B1 status.

Clinical Use

This profile is clinically useful for:

• Assessing functional vitamin B1 deficiency or insufficiency
• Investigating fatigue, neurological symptoms, concentration or memory issues
• Evaluating mitochondrial dysfunction and carbohydrate metabolism
• Monitoring patients with high metabolic demand, chronic stress, alcohol use, or malabsorption

Low TDP may be present even when free thiamine is within range, making this multi-parameter assessment clinically relevant.

Method

Analysis is performed using LC–MS/MS, allowing precise separation and quantification of individual thiamine vitamers.

Sample Requirement

• Whole blood (EDTA)
  Light protection and refrigerated transport are recommended.

Suitable for postal submission.